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non competitive sandwich elisa  (Thermo Fisher)


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    Structured Review

    Thermo Fisher non competitive sandwich elisa
    Pro-collagen I ( A ) and elastin ( B ) levels measured by <t>ELISA</t> assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).
    Non Competitive Sandwich Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15003 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non competitive sandwich elisa/product/Thermo Fisher
    Average 99 stars, based on 15003 article reviews
    non competitive sandwich elisa - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo"

    Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

    Journal: Pharmaceutics

    doi: 10.3390/pharmaceutics17010138

    Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).
    Figure Legend Snippet: Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).

    Techniques Used: Enzyme-linked Immunosorbent Assay, Control, Comparison

    Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.
    Figure Legend Snippet: Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.

    Techniques Used: Enzyme-linked Immunosorbent Assay, Comparison, Control



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    Image Search Results


    Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).

    Journal: Pharmaceutics

    Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

    doi: 10.3390/pharmaceutics17010138

    Figure Lengend Snippet: Pro-collagen I ( A ) and elastin ( B ) levels measured by ELISA assay in HFF cells treated with ZPP at concentrations of 1, 10, and 100 µg/mL. Each column represents mean ± SD. Control cells (CTRL) were arbitrarily set to 1. Data were analyzed by one-way ANOVA followed by Dunnett’s post hoc multiple comparison: p > 0.05. Pro-collagen I: F(3,13) = 2.046; p = 0.1571).Elastin: F(3,4) = 0.927; p = 0.5050. (n = 6).

    Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Control, Comparison

    Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.

    Journal: Pharmaceutics

    Article Title: Biotechnological Phytocomplex of Zanthoxylum piperitum (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo

    doi: 10.3390/pharmaceutics17010138

    Figure Lengend Snippet: Analysis of MMP-1 ( A ) and TIMP-1 ( B ) protein levels by ELISA assay in HFF cell lysate after treatment with ZPP at the concentrations of 1, 10, and 100 µg/mL for 24 h. Data were analyzed using one-way ANOVA (MMP-1: F(3,7) = 0.340, p = 0.7978; TIMP-1: F(3,7) = 1.445, p = 0.3089) followed by Dunnett’s post hoc multiple comparison ( p > 0.05). Dosage of MMP-1 ( C ) and TIMP-1 ( D ) protein levels in HFF cell lysate after pre-treatment for 2 h with ZPP at the concentrations of 1, 10, and 100 µg/mL then exposed to MetPRED (100 µg/mL) for 6 h. One-way ANOVA (MMP-1: F(4,14) = 1.551, p = 0.2450; TIMP-1: F(4,13) = 2.795, p = 0.0709) followed by Tukey’s post hoc multiple comparison; (a) p < 0.05 vs. CTRL, (n = 6). Data are expressed as mean ± SD. Control cells (CTRL) were arbitrarily set to 1.

    Article Snippet: Pro-collagen I, elastin, MMP-1, and TIMP-1 dosage were measured by using non-competitive sandwich ELISA (Human Pro-Collagen I α-1 Simple-Step kit ab210966, Abcam, Cambridge, UK; Human Elastin ELISA ab239433, Abcam; Human MMP-1 BMS2016-2, Afflimetrix-Thermofisher, Santa Clara, CA, USA; and Human TIMP-1 MA5-13688 (Afflimetrix-Thermofisher) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Comparison, Control